An interpenetrating HA/G/CS biomimic hydrogel via Diels-Alder click chemistry for cartilage tissue engineering

作者：Yu Feng; Cao Xiaodong^*; Zeng Lei; Zhang Qing; Chen Xiaofeng

来源：Carbohydrate Polymers, 2013, 97(1): 188-195.

DOI：10.1016/j.carbpol.2013.04.046

• Gelatin
• Hyaluronic acid
• Chondroitin sulfate
• Interpenetrating hydrogel
• Diels-Alder click chemistry

In order to mimic the natural cartilage extracellular matrix, a novel biological degradable interpenetrating network hydrogel was synthesized from the gelatin (G), hyaluronic acid (HA) and chondroitin sulfate (CS) by Diels-Alder "click" chemistry. HA was modified with furylamine and G was modified with furancarboxylic acid respectively. H-1 NMR spectra and elemental analysis showed that the substitution degrees of HA-furan and G-furan were 71.5% and 44.5%. Then the hydrogels were finally synthesized by cross-linking furan-modified HA and G derivatives with dimaleimide poly(ethylene glycol) (MAL-PEG-MAL). The mechanical and degradation properties of the hydrogels could be tuned simply through varying the molar ratio between furan and maleimide. Rheological, mechanical and degradation studies demonstrated that the Diels-Alder "click" chemistry is an efficient method for preparing high performance biological interpenetrating hydrogels. This biomimic hydrogel with improved mechanical properties could have great potential applications in cartilage tissue engineering.

• 出版日期2013-8-14
• 单位华南理工大学