Extraction, preliminary characterization and antioxidant properties of polysaccharides from the testa of Salicornia herbacea

作者：Wang Hongjun; Xu Ziheng; Li Xinguo; Sun Jian; Yao Dan; Jiang Hong^*; Zhou Tiezhong^*; Liu Yingzi; Li Jianguo; Wang Chunqiang; Wang Wanbing; Yue Rufeng

来源：Carbohydrate Polymers, 2017, 176: 99-106.

DOI：10.1016/j.carbpol.2017.17.047

• Salicornia herbacea
• Testa
• Polysaccharide
• Extraction
• Characterization
• Antioxidation

Response surface methodology and central composite design based on single-factor experiments were used to optimize the extraction parameters (i.e., extraction time, extraction temperature, and solid-liquid ratio) of polysaccharides from the testa of Salicornia herbacea (STP). The optimal conditions included a liquid-solid ratio of 57:1 (volume/mass), an extraction time of 300min and an extraction temperature of 87 degrees C. Under these conditions, the maximal yield of crude STP was 1.30 +/- 0.06%, which agreed with model predictions. Preliminary characterization of the purified acidic polysaccharide (STP II-1) by high performance liquid chromatography indicated that it was composed mainly of n-mannose, D-rhamnose, n-glucose, n-galactose, n-arabinose, glucuronic acid and galacturonic acid. The antioxidant capacity of STP II-1 was analyzed by monitoring both the scavenging rate of the 2,2-diphenyl-1-picrylhydrazyl free radical and inhibition rate of H202-induced erythrocyte hemolysis.

• 出版日期2017-11-15
• 单位北京农业职业学院