A ratiometric fluorescent probe based on a coumarin-hemicyanine scaffold for sensitive and selective detection of endogenous peroxynitrite

作者：Zhou Xin; Kwon Younghee; Kim Gyoungmi; Ryu Ji Hwan^*; Yoon Juyoung

来源：Biosensors and Bioelectronics, 2015, 64: 285-291.

DOI：10.1016/j.bios.2014.08.089

• Peroxynitrite sensor
• ONOO- sensor
• Reactive oxygen species probe
• Fluorescent chemosensor
• Cell imaging

In the study described herein, the red emitting probe CHCN, which possesses a linked coumarin-hemicyanine scaffold, was developed for detection of peroxynitrite (ONOO-) under physiological conditions. The studies show that CHCN displays a dual ratiometric and colorimetric response to ONOO- that is caused by an oxidation process. A possible mechanism of this oxidation process was proposed and confirmed by ESI-MS spectra for the first time. CHCN shown highly selective and sensitive towards ONOO- with a low limit of detection LOD (49.7 nM). Moreover, CHCN has appreciable cell permeability and, as a result, it is applicable to ratiometric detection of exogenous and endogenous ONOO- in living cells during phagocytic immune response. We anticipate that, owing to their ideal properties, probes of this type will find great use in explorations of the role played by ONOO- in biology.

• 出版日期2015-2-15
• 单位延边大学