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摘要
Study Objectives: Millions suffer from sleep disorders that often accompany severe illnesses such as major depression; a leading psychiatric disorder characterized by appetite and rapid eye movement sleep (REMS) abnormalities. Melanin-concentrating hormone (MCH) and nesfatin-1/NUCB2 (nesfatin) are strongly co - expressed in the hypothalamus and are involved both in food intake regulation and depression. Since MCH was recognized earlier as a hypnogenic factor, we analyzed the potential role of nesfatin on vigilance. %26lt;br%26gt;Design: We subjected rats to a 72 h-long REMS deprivation using the classic flower pot method, followed by a 3 h-long %26apos;rebound sleep%26apos;. Nesfatin mRNA and protein expressions as well as neuronal activity (Fos) were measured by quantitative in situ hybridization technique, ELISA and immunohistochemistry, respectively, in %26apos;deprived%26apos; and %26apos;rebound%26apos; groups, relative to controls sacrificed at the same time. We also analyzed electroencephalogram of rats treated by intracerebroventricularly administered nesfatin-1, or saline. %26lt;br%26gt;Results: REMS deprivation downregulated the expression of nesfatin (mRNA and protein), however, enhanced REMS during %26apos;rebound%26apos; reversed this to control levels. Additionally, increased transcriptional activity (Fos) was demonstrated in nesfatin neurons during %26apos;rebound%26apos;. Centrally administered nesfatin-1 at light on reduced REMS and intermediate stage of sleep, while increased passive wake for several hours and also caused a short-term increase in light slow wave sleep. %26lt;br%26gt;Conclusions: The data designate nesfatin as a potential new factor in sleep regulation, which fact can also be relevant in the better understanding of the role of nesfatin in the pathomechanism of depression.
- 出版日期2013-4-1
