Direct chemiluminescence determination of penicillin G potassium and a chemometrical optimization approach

作者：Sorouraddin Mohammad H^*; Iranifam Mortaza; Naseri Abdolhossein; Fadakar Sardroud Mohammad; Gharari Alibabalou Hossein

来源：Luminescence, 2011, 26(6): 622-628.

DOI：10.1002/bio.1285

• chemiluminescence
• penicillin G potassium
• central composite design
• CTMAB
• H2O2

A highly selective and simple chemiluminescence (CL) method for determination of penicillin G potassium (PGK) was developed. In the proposed method, CL was elicited from PGK upon its oxidation with H2O2. The light emission was enhanced in the presence of N-cetyl-N,N,N-trimethylammonium bromide (CTMAB). An experimental design, central composite design (CCD), was used to realize the optimized variables, including pH, surfactant (CTMAB) and H2O2 concentrations. Under optimum condition, the calibration graph was linear in the range 3.3 x 10(-3)-3.3 x 10(-1) mmol/L, with a detection limit of 8.8 x 10(-4) mmol/L for PGK. The precision was calculated by analysing samples containing 1.6 x 10(-1) mmol/L PGK (n = 5) and the relative standard deviation (RSD) was 1.40%. The utility of this method was demonstrated by determining PGK in pharmaceutical formulations for injection. The proposed method was validated by a reference method.

• 出版日期2011-12