Association and expression analysis of porcine HNF1A gene related to meat and carcass quality traits

作者：Kayan Autchara; Uddin Muhammad Jasim; Kocamis Hakan; Tesfaye Dawit; Looft Christian; Tholen Ernst; Schellander Karl; Cinar Mehmet Ulas^*

来源：Meat Science, 2013, 94(4): 474-479.

DOI：10.1016/j.meatsci.2013.04.015

• HNF1A
• Single nucleotide polymorphism
• mRNA and protein expression
• Methylation
• Meat quality
• Pig

The aim of this study was to investigate the association and expression of HNF1A gene as a candidate gene for meat and carcass quality traits in pigs. Statistical analysis revealed that the g.8260 A%26gt;G polymorphism significantly associated with pH 24(H), meat percentage and muscle area in the F-2 Duroc x Pietrain (DuPi, n = 313) and with pH 24(L), fat area and backfat thickness in the Pietrain (Pi, n = 110) population. HNF1A mRNA and protein expressions were higher (p %26lt; 0.05) in animals with the low post-mortem muscle pH 24(L). The promoter methylation profiling suggested that methylation was not involved on HNF1A expression regulation (p %26gt; 0.05) in animal with divergent muscle pH. In conclusion, polymorphism in porcine HNF1A gene could be used as a candidate marker to improve the meat and carcass quality traits, with the consideration of breed-specific effect.

• 出版日期2013-8