A Novel Rabbit Immunospot Array Assay on a Chip Allows for the Rapid Generation of Rabbit Monoclonal Antibodies with High Affinity

作者：Ozawa Tatsuhiko; Piao Xiuhong; Kobayashi Eiji; Zhou Yue; Sakurai Hiroaki; Andoh Tsugunobu; Jin Aishun; Kishi Hiroyuki^*; Muraguchi Atsushi

来源：PLos One, 2012, 7(12): e52383.

DOI：10.1371/journal.pone.0052383

Antigen-specific rabbit monoclonal antibodies (RaMoAbs) are useful due to their high specificity and high affinity, and the establishment of a comprehensive and rapid RaMoAb generation system has been highly anticipated. Here, we present a novel system using immunospot array assay on a chip (ISAAC) technology in which we detect and retrieve antigen-specific antibody-secreting cells from the peripheral blood lymphocytes of antigen-immunized rabbits and produce antigen-specific RaMoAbs with 10(-12) M affinity within a time period of only 7 days. We have used this system to efficiently generate RaMoAbs that are specific to a phosphorylated signal-transducing molecule. Our system provides a new method for the comprehensive and rapid production of RaMoAbs, which may contribute to laboratory research and clinical applications.

• 出版日期2012-12-26
• 单位哈尔滨医科大学