Association of tissue inhibitor of metalloproteinase gene polymorphisms and unexplained recurrent spontaneous abortions in Han Chinese couples

作者：Song, Guihong; Yan, Junhao; Zhang, Qian; Li, Guangyu; Chen, Zi-Jiang^*

来源：European Journal of Obstetrics & Gynecology and Reproductive Biology, 2014, 181: 84-88.

DOI：10.1016/j.ejogrb.2014.07.013

• Tissue inhibitor of metalloproteinase
• Single nucleotide polymorphisms
• Unexplained recurrent spontaneous abortion

Objective: To investigate the association of tissue inhibitor of metalloproteinase (TIMP) gene polymorphisms with unexplained recurrent spontaneous abortions (URSA) in a well-defined group of Han Chinese couples.
Study design: This is a case-control association study. Genomic DNA was extracted from peripheral blood samples from 84 couples with histories of three or more pregnancy losses and 69 age-matched healthy couples with at least one live birth and no histories of pregnancy loss. Polymerase chain reactions (PCRs) and sequencing with the fluorescent dye dideoxy-termination method were used to detect the rs4898 in TIMP-1, rs2277698 in TIMP-2, rs2234921 and rs5749511 in TIMP-3 and rs17035945 in TIMP-4 genotypes and allele frequencies.
Results: Neither the allele frequencies nor any of the genetic model of the five TIMP gene SNPs (i.e., TIMP-1-rs4898, TIMP-2-rs2277698, TIMP-3-rs5749511 and rs2234921, and TIMP-4-rs7035945) were significantly differences between the URSA couples and the control group.
Conclusions: No evidence was found for any associations between the TIMP-1,-2,-3, or -4 genes SNPs with URSA in this Han Chinese Han.

• 出版日期2014-10
• 单位山东大学; 山东中医药大学