Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells

作者：Yagishita Atsushi; Ueno Tasuku; Esumi Hiroyasu; Saya Hideyuki; Kaneko Kazuhiro; Tsuchihara Katsuya; Urano Yasuteru

来源：Bioconjugate Chemistry, 2017, 28(2): 302-306.

DOI：10.1021/acs.bioconjchem.6b00618

Aldehyde dehydrogenase (ALDH) is over-expressed in some subpopulations of stem cells and cancer cells. We have designed and synthesized the first selective fluorescent probe for class 3 ALDH (ALDH3A1). This probe enabled the visualization of ALDH3A1-positive cells by fluorescence microscopy as well as flow-cytometric isolation of ALDH3A1-positive viable cells from a human Caucasian esophageal squamous cell line (OE21) that heterogeneously expresses ALDH3A1.

• 出版日期2017-2