Multisite Performance Evaluation of an Enzyme-Linked Immunosorbent Assay for Detection of Giardia, Cryptosporidium, and Entamoeba histolytica Antigens in Human Stool

作者:Christy Nathaniel C V; Hencke Janice D; Escueta De Cadiz Aleyla; Nazib Forida; von Thien Heidrun; Yagita Kenji; Ligaba Segni; Haque Rashidul; Nozaki Tomoyoshi; Tannich Egbert; Herbein Joel F; Petri William A Jr*
来源:Journal of Clinical Microbiology, 2012, 50(5): 1762-1763.
DOI:10.1128/JCM.06483-11

摘要

A novel fecal antigen detection assay for fresh and frozen human samples that detects but does not differentiate Giardia spp, Cryptosporidium spp, and Entamoeba histolytica, the Tri-Combo parasite screen, was compared to three established enzyme-linked immunosorbent assays (ELISAs) at three international sites. It exhibited 97.9% sensitivity and 97.0% specificity, with positive and negative predictive values of 93.4% and 99.1%, respectively. The Tri-Combo test proved a reliable means to limit the use of individual parasite ELISAs to positive samples.

  • 出版日期2012-5