Potato native and wound periderms are differently affected by down-regulation of FHT, a suberin feruloyl transferase

作者：Jin Liqing; Cai Qing; Huang Wenlin; Dastmalchi Keyvan; Rigau Joan; Molinas Marisa; Figueras Merce; Serra Olga; Stark Ruth E^*

来源：Phytochemistry, 2018, 147: 30-48.

DOI：10.1016/j.phytochem.2017.12.011

• GC-MS
• FHT: suberin feruloyl transferase
• LC-MS
• Periderm suberin and wax
• Phellem
• Solanum tuberosum
• Potato skin
• Solid-state NMR
• Thioacidolysis

Potato native and wound healing periderms contain an external multilayered phellem tissue (potato skin) consisting of dead cells whose cell walls are impregnated with suberin polymers. The phellem provides physical and chemical barriers to tuber dehydration, heat transfer, and pathogenic infection. Previous RNAi-mediated gene silencing studies in native periderm have demonstrated a role for a feruloyl transferase (FHT) in suberin biosynthesis and revealed how its down-regulation affects both chemical composition and physiology. To complement these prior analyses and to investigate the impact of FHT deficiency in wound periderms, a bottom-up methodology has been used to analyze soluble tissue extracts and solid polymers concurrently. Multivariate statistical analysis of LC-MS and GC-MS data, augmented by solid-state NMR and thioacidolysis, yields two types of new insights: the chemical compounds responsible for contrasting metabolic profiles of native and wound periderms, and the impact of FHT deficiency in each of these plant tissues. In the current report, we confirm a role for FHT in developing wound periderm and highlight its distinctive features as compared to the corresponding native potato periderm.

• 出版日期2018-3