A novel SR protein binding site in a cis-regulatory element of HIV-1

作者：Suptawiwat Ornpreya^*; Boonarkart Chompunuch; Auewarakul Prasert

来源：Archives of Virology, 2010, 155(11): 1789-1795.

DOI：10.1007/s00705-010-0765-2

We have previously described a cis enhancing sequence (CES) in the gp41 region of the HIV-1 env gene. It could enhance HIV-1 Gag expression via both rev-dependent and CTE (constitutive transport element)-dependent pathways. We identified a functionally important and conserved region in the CES that contained a predicted binding sequence for an SR protein, ASF/SF2. We show here that ASF/SF2 bound to this sequence in an electrophoretic mobility shift assay and that the putative ASF/SF2-binding sequence was required for the enhancement of Gag expression by CES and might play a role in HIV-1 posttranscriptional regulation.

• 出版日期2010-11