Porcine Ear Skin as a Biological Substrate for in vitro Testing of Sunscreen Performance

作者：Sohn Myriam; Korn Verena; Imanidis Georgios^*

来源：Skin Pharmacology and Physiology, 2015, 28(1): 31-41.

DOI：10.1159/000358273

• Sun protection factor
• Pig ear skin
• In vitro testing
• Surface roughness
• Polymethylmethacrylate plates
• Sunscreen

Purpose: The purpose of the study was to examine the use of skin from porcine ears as a biological substrate for in vitro testing of sunscreens in order to overcome the shortcomings of the presently used polymethylmethacrylate (PMMA) plates that generally fail to yield a satisfactory correlation between sun protection factors (SPF) in vitro and in vivo. Procedures: Trypsin-separated stratum corneum and heat-separated epidermis provided UV-transparent substrates that were laid on quartz or on PMMA plates. These were used to determine surface roughness by chromatic confocal imaging and to measure SPF in vitro of 2 sunscreens by diffuse transmission spectroscopy. Results: The recovered skin layers showed a lower roughness than full-thickness skin but yielded SPF in vitro values that more accurately reflected the SPF determined in vivo by a validated procedure than PMMA plates, although the latter had in part roughness values identical to those of intact skin. Combination of skin tissue with a high roughness PMMA plate also provided accurate SPF in vitro. Conclusions: Besides roughness, the improved affinity of the sunscreen to the skin substrate compared to PMMA plates may explain the better in vitro prediction of SPF achieved with the use of a biological substrate.

• 出版日期2015