Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate

作者：Kajiya Mikihito; Silva Marcelo J B; Sato Kimihiro; Ouhara Kazuhisa; Kawai Toshihisa^*

来源：Biochemical and Biophysical Research Communications, 2009, 386(1): 11-15.

DOI：10.1016/j.bbrc.2009.05.117

• Molecular hydrogen
• Colitis
• Inflammation
• Antioxidant
• Colon
• Dextran sodium sulfate
• IL-12
• TNF-alpha
• IL-1-beta
• Macrophages

By its antioxidant effect, molecular hydrogen gas (H(2)) was reported to protect organs from tissue damage induced by ischemia reperfusion. To evaluate its anti-inflammatory effects, we established a mouse model of human inflammatory bowel disease (IBD) by supplying mice with water containing (1) dextran sodium sulfate (DSS) (5%), (2) DSS (5%) and H(2), or (3) H(2) only ad libitum up to 7 days. At day-7, DSS-induced pathogenic outcomes including, loss of body weight, increase of colitis score, pathogenic shortening of colon length, elevated level of IL-12. TNF-alpha and IL-1 beta in colon lesion, were significantly suppressed by the addition of H(2) to DSS solution. Histological analysis also revealed that the DSS-mediated colonic tissue destruction accompanied by macrophage infiltration was remarkably suppressed by H(2). Therefore, the present study indicated that H(2) can prevent the development of DSS-induced colitis in mice.

• 出版日期2009-8-14