A sensitive and selective LC-MS/MS method for quantitation of ivermectin in human, mouse and monkey plasma: clinical validation

作者：Chhonker, Yashpal S.; Ma, Liping; Edi, Constant; Murry, Daryl J.^*

来源：Bioanalysis, 2018, 10(22): 1841-1852.

DOI：10.4155/bio-2018-0110

• abamectin
• ivermectin
• LC-MS/MS
• pharmacokinetics

Aim: A sensitive and selective LC-MS/MS method was validated for quantitation of ivermectin (IVM) in plasma. Method: The IVM was extracted from plasma using solid-phase extraction with C-18 cartridges. Separation of analytes was achieved on an ACE C18 column with isocratic elution using 0.1% acetic acid and methanol: acetonitrile (1: 1, v/v) as mobile phase. The IVM was quantitated using electrospray ionization operating in negative multiple reaction monitoring mode. Results: The MS/MS response was linear over the concentration range from 0.1-1000 ng/ml. The method for human plasma was validated as per US FDA guidelines. The LC-MS/MS method is sensitive, reproducible, has easy sample preparation and is suitable for IVM quantitation in clinical samples.

• 出版日期2018-11
• 单位北京大学