Understanding gene functions and disease mechanisms: Phenotyping pipelines in the German Mouse Clinic

作者：Fuchs Helmut; Aguilar Pimentel Juan Antonio; Amarie Oana V; Becker Lore; Calzada Wack Julia; Cho Yi Li; Garrett Lillian; Hoelter Sabine M; Irmler Martin; Kistler Martin; Kraiger Markus; Mayer Kuckuk Philipp; Moreth Kristin; Rathkolb Birgit; Rozman Jan; Buttkus Patricia da Silva; Treise Irina; Zimprich Annemarie; Gampe Kristine; Hutterer Christine; Stoeger Claudia; Leuchtenberger Stefanie; Maier Holger; Miller Manuel; Scheideler Angelika; Wu Moya; Beckers Johannes

来源：Behavioural Brain Research, 2018, 352: 187-196.

DOI：10.1016/j.bbr.2017.09.048

• Mouse phenotyping
• Phenotyping pipeline
• Mouse model
• Gene function analysis

Since decades, model organisms have provided an important approach for understanding the mechanistic basis of human diseases. The German Mouse Clinic (GMC) was the first phenotyping facility that established a collaboration-based platform for phenotype characterization of mouse lines. In order to address individual projects by a tailor-made phenotyping strategy, the GMC advanced in developing a series of pipelines with tests for the analysis of specific disease areas. For a general broad analysis, there is a screening pipeline that covers the key parameters for the most relevant disease areas. For hypothesis-driven phenotypic analyses, there are thirteen additional pipelines with focus on neurological and behavioral disorders, metabolic dysfunction, respiratory system malfunctions, immune-system disorders and imaging techniques. In this article, we give an overview of the pipelines and describe the scientific rationale behind the different test combinations.

• 出版日期2018-10-15