A new point mutation in exon 17 of the horse (Equus caballus) RYR1 gene (short communication)

作者：Gronek P^*; Plawski A; Nuc K; Nowicka Posluszna A; Slomski R

来源：Czech Journal of Animal Science, 2000, 45(10): 443-445.

• equine genetics
• RYR1 gene
• stress susceptibility

The polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), and sequencing analysis were used for the study of the ryanodine receptor gene (RYRI) of Wielkopolska horses, Thoroughbred, and Pure Arabian. PCR-amplified RYRI gene specific fragments were cloned in pGem5 Zf(-) plasmid. Double stranded templates were sequenced by the Sanger dideoxy chain termination method, using Cy-5' labeled primers. The analyzed region of the RYRI gene was characterized by a high homology (97.3-98.7%) between all breeds. We detected transversion G-->T and as a consequence a substitution of alanine for serine. We did not detect any 1843C-->T mutation in the equine RYR1 gene.

• 出版日期2000-10