High-performance liquid chromatography purification of chemically modified RNA aptamers

作者：Lin Chi Yen; Huang Zhen; Jaremko William; Niu Li^*

来源：Analytical Biochemistry, 2014, 449: 106-108.

DOI：10.1016/j.ab.2013.12.022

• RNA purification and separation
• HPLC
• Chemically modified RNA

2'-Fluoro modified RNAs are useful as potential therapeutics and as special substrates for studying RNA function. 2'-Fluoro modified RNAs generally need to be purified after they are prepared either enzymatically or by solid-phase synthesis. Here we introduce a protocol by which 2'-fluoro modified RNAs with 57 and 58 nucleotides can be resolved and purified using ion-pair, reverse-phase high-performance liquid chromatography (HPLC). Because the size of our RNA samples is in the range of many known RNA aptamers of therapeutic values, our protocol should be generally useful.

• 出版日期2014-3-15