Determination of amino acids in selenium-enriched yeast by gas chromatography mass spectrometry after microwave assisted hydrolysis

作者：Zhang Xu; Yang Lu; Mester Zoltan^*

来源：Analytica Chimica Acta, 2012, 744: 54-59.

DOI：10.1016/j.aca.2012.07.011

• Amino acids
• Microwave-irradiation hydrolysis
• Gas chromatography-mass spectrometry
• Selenium enriched yeast

A simple, rapid microwave digestion procedure for protein hydrolysis preceding the determination of amino acids in yeast using gas chromatography-mass spectrometry (GC-MS) is described. Protein hydrolysis was performed in a focused microwave using 4 M methanesulfonic acid (MAS). Amino acids were derivatized with methyl chlorofomate (MCF) and extracted into chloroform prior to GC-MS analysis. The microwave parameters, including power, temperature and heating time, were optimized. It was found that temperature and heating time were the most influential factors. A total of 17 amino acids were determined in selenium-enriched yeast with use of standard addition calibration. Limits of detection and quantitation (LODs/LOQs) of the amino acids measured were in the sub-nmol range, suitable for monitoring of amino acids in yeast and other food products.

• 出版日期2012-9-26