DL-3-n-Butylphthalide protects rat bone marrow stem cells against hydrogen peroxide-induced cell death through antioxidation and activation of PI3K-Akt pathway

作者：Sun Bo; Feng Meijiang; Tian Xiangyang; Lu Xiaowei; Zhang Yunyun; Ke Xianjin; Huang Susu; Cao Jingjing; Ding Xinsheng^*

来源：Neuroscience Letters, 2012, 516(2): 247-252.

DOI：10.1016/j.neulet.2012.04.003

• DL-3-n-Butylphthalide
• Oxidative stress
• Bone marrow stem cells
• Antioxidant
• PI3K/Akt

Bone marrow stem cells (BMSCs) have been one of the most important cell sources for cell replacement therapy (CRT) in cerebral infarction. However, long-lasting oxidative stress during stroke, which plays an important role in neuron damage, deteriorates the microenvironment for cell survival, differentiation and removal. Thus the outcome of CRT in ischemic diseases was poor. DL-3-n-Butylphthalide (NBP) has protective effects on ischemic brain tissue through multiple mechanisms and has been used for stroke treatment in China for several years. In this study, hydrogen peroxide (H2O2) was used to induce oxidative stress injury to rat bone marrow stem cells (rBMSCs), imitating the microenvironment surrounding transplanted cells in the ischemic brain in vitro. The protective effects of NBP on rBMSCs against apoptosis induced by oxidative stress were investigated. Our results indicated that NBP could protect rBMSCs against apoptosis due to antioxidative properties and modulation of PI3K/Akt pathway. NBP could be used in combination with BMSCs for the treatment of cerebral infarction by improving the oxidative stress microenvironments and cell survival, however, further studies remain warranted.

• 出版日期2012-5-16
• 单位南京医科大学