Simultaneous injection effective mixing flow analysis of urinary albumin using dye-binding reaction

作者：Ratanawimarnwong Nuanlaor; Ponhong Kraingkrai; Teshima Norio^*; Nacapricha Duangjai; Grudpan Kate; Sakai Tadao; Motomizu Shoji

来源：Talanta, 2012, 96: 50-54.

DOI：10.1016/j.talanta.2012.02.027

• Simultaneous injection effective mixing analysis (SIEMA)
• Tetrabromophenol blue
• Albumin
• Urine

A new four-channel simultaneous injection effective mixing flow analysis (SIEMA) system has been assembled for the determination of urinary albumin. The SIEMA system consisted of a syringe pump, two 5-way cross connectors, four holding coils, five 3-way solenoid valves, a 50-cm long mixing coil and a spectrophotometer. Tetrabromophenol blue anion (TBPB) in Triton X-100 micelle reacted with albumin at pH 3.2 to form a blue ion complex with a lambda(max) 625 nm. TBPB, Triton X-100, acetate buffer and albumin standard solutions were aspirated into four individual holding coils by a syringe pump and then the aspirated zones were simultaneously pushed in the reverse direction to the detector flow cell. Baseline drift, due to adsorption of TBPB-albumin complex on the wall of the hydrophobic PTFE tubing, was minimized by aspiration of Triton X-100 and acetate buffer solutions between samples. The calibration graph was linear in the range of 10-50 mu g/mL and the detection limit for albumin (3 sigma) was 0.53 mu g/mL. The RSD (n = 11) at 30 mu g/mL was 1.35%. The sample throughput was 37/h. With a 10-fold dilution, interference from urine matrix was removed. The proposed method has advantages in terms of simple automation operation and short analysis time.

• 出版日期2012-7-15