A DOG GROWTH-HORMONE CDNA CODES FOR A MATURE PROTEIN IDENTICAL TO PIG GROWTH-HORMONE

作者：ASCACIOMARTINEZ JA; BARRERASALDANA HA

来源：Gene, 1994, 143(2): 277-280.

DOI：10.1016/0378-1119(94)90110-4

• CANIS FAMILIARIS
• PITUITARY GLAND
• POLYMERASE CHAIN REACTION
• NUCLEOTIDE SEQUENCE
• BACTERIAL EXPRESSION

Although methods for purification of dog (Canis familial is) growth hormone (cfGH) were described in the late Sixties, the cloning of its cDNA has not been achieved until now. In order to clone the cfGH cDNA, we capitalized on the high degree of nucleotide sequence conservation among mammalian GH genes to design a pair of consensus oligodeoxyribonucleotide primers. With these, and starting with dog pituitary gland total RNA, we specifically amplified the cfGH cDNA using the reverse transcription-polymerase chain reaction. Its coding sequence (651 bp), as well as its 3' untranslated region (101 bp), resemble those of a typical mammalian GH cDNA. Interestingly, its encoded mature protein is identical to pig growth hormone (pGH).

• 出版日期1994-6-10