Deoxyschizandrin Loaded Liposomes on the Suppression Lipid Accumulation in 3T3-L1 Adipocytes

作者：Liu, Xiaona; Wang, Shifeng; Wu, Zhisheng^*; Wang, Zhaoyi; Zheng, Qiusheng; Li, Defang^*

来源：Molecules, 2018, 23(9): 2158.

DOI：10.3390/molecules23092158

• deoxyschizandrin
• nonalcoholic fatty liver disease
• liposomes
• lipid droplet
• 3T3-L1 adipocyte

Deoxyschizandrin (DS) is a bioactive benzocyclooctadiene lignan found in the fruit of Schisandra chinensis. However, poor bioavailability and non-specificity of DS frequently caused low therapeutic efficacy. In the present study, DS-liposome (DS-lipo) was implemented to enhance the hepatic targeting and inhibition effects on adipocyte differentiation in 3T3-L1 cells. The formulations enabled encapsulation of as much as 24.14% DS. The DS-lipo prepared was about 73.08 nm, as measured by laser light scattering (LLS) morphology. In the visual field of a scanning electron microscope (SEM), the liposomes were spherical with similar size and uniform dispersion. Fluorescence live imaging study exhibited hepatic targeting of liposomes in vivo. Furthermore, High-Content Analysis (HCS) imaging microassay analyses revealed DS-lipo and DS reduced cytoplasmic lipid droplet in 3T3-L1 adipocytes, with the IC50 value of 8.68 mu M and 31.08 mu M, respectively. The lipid droplet accumulation inhibition rate of 10 mu M DS-lipo was above 90%, which was even superior to the effect of 30 mu M DS solution. The current findings suggest that DS-lipo was a therapeutic strategy for alleviating lipid-associated diseases and nonalcoholic fatty liver disease (NAFLD).

• 出版日期2018-9
• 单位北京中医药大学; 滨州医学院