Abscission-related genes revealed by RNA-Seq analysis using self-abscising apple (Malus x domestica)

作者：Heo S; Hwang J H; Jun J H; Lee H J^*

来源：Journal of Horticultural Science & Biotechnology, 2016, 91(3): 271-278.

DOI：10.1080/14620316.2016.1155315

• Malus x domestica
• apple
• RNA-seq
• self-abscission

The self-abscising characteristic of apple (Malus x domestica) fruit is a prominent laboursaving trait during cultivation. Early abscission up to 30 d after full bloom leaves only the central fruit in a cluster, while the four lateral fruit are abscised. A comparative analysis was conducted between the central and lateral pedicels in the self-abscising apple cultivar 'Saika' to identify the genes that trigger the abscission mechanism, as the destinies of the pedicels in a cluster are obvious. Transcriptome analysis was performed using RNA-Seq to compare expression profiles between the surviving central pedicel and the abscised lateral pedicels. A total of 797,647 expressed sequence tags were assembled into 65,876 contigs, which were annotated and analysed using Blast2GO. A total of 1,585 differentially expressed genes were identified in the central and lateral pedicels using NOISeq software. Differentially expressed genes were confirmed by quantitative real-time reverse-transcription polymerase chain reaction analysis, focussing on the genes associated with auxin signalling. Our data suggested that the IAA14/SLR gene is at least partly involved in the mechanism of abscission of the lateral pedicels.

• 出版日期2016