Analytical potential of mid-infrared detection in capillary electrophoresis and liquid chromatography A review

作者：Kuligowski Julia; Quintas Guillermo; de la Guardia Miguel; Lendl Bernhard^*

来源：Analytica Chimica Acta, 2010, 679(1-2): 31-42.

DOI：10.1016/j.aca.2010.09.006

• Liquid chromatography
• Capillary electrophoresis
• Fourier transform infrared spectrometry
• Hyphenated techniques

Literature published in the last decade concerning the use of mid-infrared spectrometry as a detection system in separation techniques employing a liquid mobile phase is reviewed In addition to the continued use of isocratic liquid chromatographic (LC) techniques advances in chemometric data evaluation techniques now allow the use of gradient techniques on a routine basis thus significantly broadening the range of possible applications of LC-IR. The general trend towards miniaturized separation systems was also followed for mid-IR detection where two key developments are of special importance Firstly concerning on-line detection the advent of micro-fabricated flow-cells with Inner volumes of only a few nL for transmission as well as attenuated total reflection measurements enabled on-line mid-IR detection in capillary LC and opened the path for the first successful realization of on-line mid-IR detection in capillary zone electrophoresis as well as micellar electrokinetic chromatography Secondly concerning off-line detection the use of micro-flow through dispensers now enables to concentrate eluting analytes on dried spots sized a few tens of micrometers thus matching the dimensions for sensitive detection by mid-IR microscopy Finally in an attempt to increase detection sensitivity of on-line mid-IR detection mid-IR quantum cascade lasers have been used Applications cover the field of food analysis environmental analysis and the characterization of explosives among others Best detec

• 出版日期2010-10-29