Depth resolved hyperspectral imaging spectrometer based on structured light illumination and Fourier transform interferometry

作者：Choi Heejin^*; Wadduwage Dushan; Matsudaira Paul T; So Peter T C

来源：Biomedical Optics Express, 2014, 5(10): 3494-3507.

DOI：10.1364/BOE.5.003494

A depth resolved hyperspectral imaging spectrometer can provide depth resolved imaging both in the spatial and the spectral domain. Images acquired through a standard imaging Fourier transform spectrometer do not have the depth-resolution. By post processing the spectral cubes (x, y, lambda) obtained through a Sagnac interferometer under uniform illumination and structured illumination, spectrally resolved images with depth resolution can be recovered using structured light illumination algorithms such as the HiLo method. The proposed scheme is validated with in vitro specimens including fluorescent solution and fluorescent beads with known spectra. The system is further demonstrated in quantifying spectra from 3D resolved features in biological specimens. The system has demonstrated depth resolution of 1.8 mu m and spectral resolution of 7 nm respectively.

• 出版日期2014-10-1