A comprehensive profile of circulating RNAs in human serum

作者：Umu Sinan Ugur^*; Langseth Hilde; Bucher-Johannessen Cecilie; Fromm Bastian; Keller Andreas; Meese Eckart; Lauritzen Marianne; Leithaug Magnus; Lyle Robert; Rounge Trine B.

来源：RNA Biology, 2018, 15(2): 242-250.

DOI：10.1080/15476286.2017.1403003

• Small RNA
• rna sequencing
• serum
• circulating RNA
• RNA fragments
• Bioinformatics
• cancer

Non-coding RNA (ncRNA) molecules have fundamental roles in cells and many are also stable in body fluids as extracellular RNAs. In this study, we used RNA sequencing (RNA-seq) to investigate the profile of small non-coding RNA (sncRNA) in human serum. We analyzed 10billion Illumina reads from 477 serum samples, included in the Norwegian population-based Janus Serum Bank (JSB). We found that the core serum RNA repertoire includes 258micro RNAs (miRNA), 441 piwi-interacting RNAs (piRNA), 411 transfer RNAs (tRNA), 24 small nucleolar RNAs (snoRNA), 125 small nuclear RNAs (snRNA) and 123 miscellaneous RNAs (misc-RNA). We also investigated biological and technical variation in expression, and the results suggest that many RNA molecules identified in serum contain signs of biological variation. They are therefore unlikely to be random degradation by-products. In addition, the presence of specific fragments of tRNA, snoRNA, Vault RNA and Y_RNA indicates protection from degradation. Our results suggest that many circulating RNAs in serum can be potential biomarkers.

• 出版日期2018