Microchip device for cell lysis, multiplex PCR amplification, and electrophoretic sizing

作者：Waters LC; Jacobson SC; Kroutchinina N; Khandurina J; Foote RS; Ramsey JM^*

来源：Analytical Chemistry, 1998, 70(1): 158-162.

DOI：10.1021/ac970642d

The steps of cell lysis, multiplex PCR amplification, and electrophoretic analysis are executed sequentially on a monolithic microchip device, The entire microchip is thermally cycled to lyse cells and to amplify DNA, and the products are then analyzed using a sieving medium for size separation and an intercalating dye for fluorescence detection. Using a standard PCR protocol, a 500-base pair (bp) region of bacteriophage lambda DNA and 154-, 264-, 346-, 410-, and 550-bp regions of E. coli genomic and plasmid DNAs are amplified. The electrophoretic analysis of the products is executed in <3 min following amplification using hydroxyethyl cellulose or poly(dimethylacryl-amide) sieving gels. Product sizing is demonstrated by proportioning the amplified product with a DNA sizing ladder.

• 出版日期1998-1-1