Dopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase Candida sp 99-125

作者：Gao, Junkai; Jiang, Yanjun; Lu, Jinshu; Han, Zhi; Deng, Jiajia; Chen, Yan^*

来源：Scientific Reports, 2017, 7(1): 40395.

DOI：10.1038/srep40395

• ENZYME IMMOBILIZATION
• EFFICIENT REMOVAL
• RUGOSA LIPASE
• FACILE
• STABILIZATION
• MICROSPHERES
• STABILITY
• BIODIESEL
• SBA-15

Dopmine functionalized mesoporous onion-like silica (DPMS) was synthesized via a biomimetic coating, and lipase Candida sp. 99-125 (LCS) was immobilized in DPMS (LCS@DPMS) by physical adsorption in this study. The DPMS was characterized by SEM, TEM, BET and FT-IR, and it was shown that the DPMS had clear multishell structures with large surface area of 419 m(2)/g. The activity, pH stability, thermal stability, storage stability, and reusability of the LCS@DPMS were investigated in detail. The stabilities of LCS@DPMS were improved significantly compared to the free lipase and LCS@MS (LCS immobilized in unfunctionalized mesoporous onion-like silica by physical adsorption). All the results indicated that the DPMS had high efficiency and improved stability for lipase immobilization.

• 出版日期2017-1-9
• 单位浙江海洋大学; 河北工业大学