Acaricidal activity of four fractions and octadecanoic acid-tetrahydrofuran-3,4-diyl ester isolated from chloroform extracts of neem (Azadirachta indica) oil against Sarcoptes scabiei var. cuniculi larvae in vitro

作者：Du Yong Hua; Li Jin Liang; Jia Ren Yong; Yin Zhong Qiong^*; Li Xu Ting; Lv Cheng; Ye Gang; Zhang Li; Zhang Yu Qun

来源：Veterinary Parasitology, 2009, 163(1-2): 175-178.

DOI：10.1016/j.vetpar.2009.04.002

• Octadecanoic acid-tetrahydrofuran-3,4-diyl ester
• Neem oil
• Sarcoptes scabiei
• Acaricidal activity

Four fractions obtained from chloroform extracts of neem (Azadirachta indica) oil by column chromatography were investigated for acaricidal activity against Sarcoptes scabiei var. cuniculi larvae in vitro. Octadecanoic acid-tetrahydrofuran-3,4-diyl ester was isolated from an active fraction of the chloroform extract and its toxicity against S. scabiei larvae was tested in vitro. A complementary log-log model was used to analyse the toxicity data. Activity was found in the third fraction, with 100% corrected mortality after 4.5 h of exposure at a concentration of 200 mg ml(-1). This fraction was repeatedly re-crystallised in acetone to yield a white amorphous powder, identified as octadecanoic acid-tetrahydrofuran-3,4-diyl ester, with a median lethal concentration (LC(50)) Of 0-1 mg ml(-1) at 24 h post-treatment. The median lethal time (LT(50)) for this compound was 15.3 h at a concentration of 7.5 mg ml(-1).

• 出版日期2009-7-7
• 单位四川农业大学; 四川省畜牧科学研究院; 宜宾学院