A simple and high-throughput method of ultrasonic extraction-capillary electrophoresis for determination of melamine in milk

作者：Lv Yun Kai^*; Sun Yue Na; Wang Li Min; Jia Cui Ling; Sun Han Wen

来源：Analytical Methods, 2011, 3(11): 2557-2561.

DOI：10.1039/c1ay05426k

A simple, rapid, sensitive, and low cost method of ultrasonic extraction (USE) and capillary electrophoresis (CE) for the determination of melamine in milk is developed. The most widely used extraction methods of melamine (SPE and LLE) were compared with ultrasonic-assisted extraction by evaluating simplicity, cost and sample clean-up effect, and the conditions of CE were optimized. The melamine was extracted by acetonitrile under the action of ultrasonic energy. After centrifuging, diluting and filtering, the extract was analyzed by CE directly. The determination used 20 mM NaH(2)PO(4) as running buffer (pH 2.6), UV detection at 208 nm, and an applied voltage of 18 kV. The proposed method demonstrated good performance concerning linearity (R(2) = 0.9989), precision (2.6-4.8%), limit of detection and quantification (LOD, 0.163 mg kg(-1); LOQ, 0.542 mg kg(-1)) and accuracy (85.1-98.3%). The proposed method is rapid, convenient and low-cost for the determination of melamine in milk products.

• 出版日期2011-11
• 单位河北大学