A self-contained polymeric 2-DE chip system 2 for rapid and easy analysis

作者：Usui Keisuke; Hiratsuka Atsunori; Shiseki Kisho; Maruo Yuji; Matsushima Toshiyuki; Takahashi Katsuyoshi; Unuma Yutaka; Sakairi Koji; Namatame Ichiji; Ogawa Yasuhiro; Yokoyama Kenji^*

来源：Electrophoresis, 2006, 27(18): 3635-3642.

DOI：10.1002/elps.200600221

• 2-DE
• miniaturization
• polymeric device
• protein separation
• self-contained device

We developed a polymeric 2-DE chip system. The chip consisted of an IEF region, an SDS-PAGE region, a valveless connection port, and a sample introduction port. A "junction structure" as a valveless connection port, which allowed separating and connecting the first- and second-dimensional gels, was fabricated between their regions. A "solution inlet" as a sample introduction port was fabricated to perform the liquid and sample introductions without solution leakage. Simultaneous sample monitoring was performed using the on-chip detection system. The performances of the system were demonstrated using commercially available proteins as a standard specimen and tissue-extracted proteins as the real samples. All procedures were employed without any movement of relocation part. This new 2-D separation system realized improved labor-intensive operations and a reduced experimental time.

• 出版日期2006-9