Arabinogalactan protein from Arachis hypogaea: Role as carrier in drug-formulations

作者：Parveen Seema; Gupta Ashish D; Prasad Ramasare^*

来源：International Journal of Pharmaceutics, 2007, 333(1-2): 79-86.

DOI：10.1016/j.ijpharm.2006.10.003

• arabinogalactan protein
• Yariv reagent
• Candida albicans
• amphotericin-B

Arabinogalactanl protein (AGP) a highly water-soluble glyco-conjugate from groundnut (Arachis hypogaea L.) seedling was isolated and purified by precipitation with P-glucosyl Yariv reagent. Quantification of AGP was done by gel diffusion assay. Purified AGP was conjugated to amphotericin-B (AmB) by Schiff base reaction at pH 11.0, with aim to prepare a water-injectable lesser toxic AGP-AmB conjugate without affecting AmB antifungal potential. The AGP-AmB conjugate antifungal activity was assayed by serial broth dilution and disc method against several Candida albicans clinical isolates. Both AmB and AGP-AmB showed similar MICs and MFCs activities, indicating that AGP do not reduced the antifungal activity of AmB. However, the in vitro and in vivo toxicity assays revealed that AGP-AmB conjugate was lesser toxic than AmB, as high MTD (45 mg/kg body weight) was observed. It is suggested that AGP could be a potent carrier in AmB formulation, which may result in effective treatment of fungal infections.

• 出版日期2007-3-21