Ni2 -PAA adsorbent for purifying 6His-OmpTS recombinant protein

作者：Zeng, QB^*; Ye, QZ; Xu, JR; Fu, RW

来源：Chinese Chemical Letters, 2002, 13(5): 436-439.

• affinity matrix
• polyacrylic acid
• chelate
• nickel ion
• hexa-histidine tagged

Functional Ni2 -polyacrylic acid (Ni2 -PAA) adsorbent has been prepared for metal chelate affinity chromatography. DNA elements coding for adjacent histidines were fused to the Aeromonas hydrophila ompTS gene. Subsequent expression in E. coli resulted in the production of hybrid protein 6His-OmpTS that could be purified by Ni2 -PAA affinity chromatography.

• 出版日期2002-5
• 单位中山大学; 南方医科大学