Alpha-synuclein immunoreactivity patterns in the enteric nervous system

作者：Aldecoa Iban; Navarro Otano Judith; Stefanova Nadia; Sprenger Fabienne S; Seppi Klaus; Poewe Werner; Cuatrecasas Miriam; Valldeoriola Francesc; Gelpi Ellen; Tolosa Eduard^*

来源：Neuroscience Letters, 2015, 602: 145-149.

DOI：10.1016/j.neulet.2015.07.005

• Parkinson disease
• Alpha-synuclein
• Enteric nervous system
• Gastrointestinal tract
• Lewy pathology

We aimed to compare immunoreactivity patterns of four different anti-alpha-syn antibodies in surgical specimens of the gastrointestinal tract of Parkinson disease and control cases.
Surgical specimens from stomach, small and large bowel of 6 PD cases and 12 controls were studied. Primary antibodies: anti-a-syn clone KM51, anti-phosphorylated alpha-syn Ser129, anti-alpha-syn clone 15G7 and anti-nitrated alpha-syn505. We found different immunoreactivity patterns: (a) coarse, Lewy-body-like aggregates labelled by the 4 antibodies and detected in 4/6 PD cases and in 1/12 controls; (b) distinct punctate cytoplasmic staining of ganglion cells labelled by anti-phosphorylated-alpha-syn and detected in 3/6 PD cases and 3/12 controls; (c) fine diffuse, synaptic-type staining of neural structures labelled by anti-alpha-syn-15G7 and anti-nitrated-alpha-syn505 and detected in all subjects.
We conclude that different specific and non-specific immunoreactivity patterns are detected in surgical specimens of gastrointestinal tract when using different anti-a-syn antibodies, as they recognize different epitopes and states of alpha-synuclein protein. Coarse aggregates in neural structures seem to be the most promising marker for the diagnosis of Lewy-body parkinsonism when evaluating abnormal a-syn in the gastrointestinal tract.

• 出版日期2015-8-18