Altered expression of adhesion molecules on peripheral blood leukocytes in feline infectious peritonitis

作者：Olyslaegers Dominique A J; Dedeurwaerder Annelike; Desmarets Lowiese M B; Vermeulen Ben L; Dewerchin Hannah L; Nauwynck Hans J^*

来源：Veterinary Microbiology, 2013, 166(3-4): 438-449.

DOI：10.1016/j.vetmic.2013.06.027

• FIP
• Adhesion molecules
• Peripheral blood leukocytes
• Flow cytometry

Feline infectious peritonitis (FIP) is a fatal, coronavirus-induced systemic disease in domestic and wild felids. The pathology associated with FIP (multifocal granulomatous vasculitis) is considered to be elicited by exaggerated activation and subsequent extravasation of leukocytes. As changes in the expression of adhesion molecules on circulating leukocytes precede their margination and emigration, we reasoned that the expression of leukocyte adhesion molecules may be altered in FIP. In present study, the expression of principal adhesion molecules involved in leukocyte transmigration (CD15s, CD11a, CD11b, CD18, CD49d, and CD54) on peripheral blood leukocytes from cats with naturally occurring FIP (n = 15) and controls (n = 12) was quantified by flow cytometry using a formaldehyde-based rapid leukocyte preparation technique. T- and B-lymphocytes from FIP patients exhibit higher expression of both subunits (CD11a and CD18) composing the 132 integrin lymphocyte function-associated antigen (LFA)-1. In addition, the expression of the alpha(4) subunit (CD49d) of the 131 integrin very late antigen (VLA)-4 was elevated on B-lymphocytes from FIP patients. The expression of CD11b and CD18, that combine to form the 132 integrin macrophage-1 antigen (Mac-1), was elevated on monocytes, whereas the density of CD49d was reduced on this population in FIP. Granulocytes of FIP cats displayed an increased expression of the a chain of Mac-1 (CD11b). These observations suggest that leukocytes from FIP patients show signs of systemic activation causing them to extravasate into surrounding tissues and ultimately contribute to pyogranuloma formation seen in FIP.

• 出版日期2013-10-25