Novel Terpenoids, Trichoderonic Acids A and B Isolated from Trichoderma virens, Are Selective inhibitors of Family X DNA Polymerases

作者：Yamaguchi Yasuhiro; Manita Daisuke; Takeuchi Toshifumi; Kuramochi Kouji; Kuriyama Isoko; Sugawara Fumio; Yoshida Hiromi; Mizushina Yoshiyuki^*

来源：Bioscience Biotechnology and Biochemistry, 2010, 74(4): 793-801.

DOI：10.1271/bbb.90843

• trichoderonic acid A
• trichoderonic acid B
• terpenoid
• family X of DNA polymerases
• enzyme inhibitor

Trichoderonic acids A (1) and B (2), novel terpenoids, and (+)-heptelidic acid (3) isolated from cultures of a fungus, Trichoderma virens, and their structures were identified by spectroscopic analysis. These compounds selectively and competitively inhibited the activities of mammalian DNA polymerases beta, lambda (pols beta, lambda), and terminal deoxynucleotidyl transferase (TdT) in family X of pots, and compound 2 was a stronger inhibitor than compound 1 or 3. On the other hand, compounds 1-3 did not influence the activities of the other families (A-, B-, and the Y-families) of the mammalian pols tested, and showed no effect on the activities of plant pol alpha, fish pol delta, prokaryotic pol, or the other DNA metabolic enzymes tested. Compound 2 suppressed the growth of two human cancer cell lines, cervix carcinoma cells (Ham) and breast carcinoma cells (MCF-7). The results suggest that these compounds identified inhibition among the families of mammalian pols.

• 出版日期2010-4