Amino acid tissue levels and GABA(A) receptor binding in the developing rat cerebellum following status epilepticus

作者：Lopez Meraz Maria Leonor^*; Rocha Luisa L; Miquel Marta; Ortega Jenny C; Perez Estudillo Cesar A; Garcia Luis I; Hernandez M E; Manzo J

来源：Brain Research, 2012, 1439: 82-87.

DOI：10.1016/j.brainres.2011.12.038

• Status epilepticus
• Developing rat
• Cerebellum
• GABA(A) receptor
• Amino acids

Incidence of status epilepticus (SE) is higher in children than in adults and SE can be induced in developing rats. The cerebellum can be affected after SE; however, consequences of cerebellar amino acid transmission have been poorly studied. The goal of this study was to determine amino acid tissue concentration and GABA(A) receptor binding in the immature rat cerebellum after an episode of SE. Thirteen-day-old (P13) rat pups received intraperitoneal injections of lithium chloride (3 mEq/kg). Twenty hours later, on P14, SE was induced by subcutaneous injection of pilocarpine hydrochloride (60 mg/kg). Control animals were given an equal volume of saline subcutaneously. Animals were killed 24 h after SE induction, the cerebellum was quickly removed, and the vermis and hemispheres were rapidly dissected out on ice. Amino acid tissue concentrations in the vermis and hemispheres were evaluated by HPLC and fluorescent detection. GABA(A) receptor binding in the medial vermis was analyzed by in vitro autoradiography. SE increased the tissue levels of the inhibitory amino acids taurine (80%) and alanine (91%), as well as glutamine (168%) in the cerebellar hemisphere; no changes were observed in the vermis. SE did not modify GABA(A) receptor binding in any cerebellar lobule from the vermis. Our data demonstrate that SE produces region-specific changes in amino acid concentrations in the developing cerebellum.

• 出版日期2012-2-23