A preliminary study of purification and bioactivity of a 12 600 shark cartilage angiogenesis inhibitor

作者：Zeng, F^*; Xie, TX; Yang, WX; Zhang, ZX; Yu, JX; Pang, Y

来源：Chemical Journal of Chinese Universities, 2001, 22(9): 1462-1465.

• shark cartilage
• protein
• purification
• bioactivity

The purification method and bioactivity of shark cartilage angiogenesis inhibitor-a (SCAI-a) from Guangdong Yangjiang were investigated. The 1.0 mol/L guanidine and 0.02 mol/L MES(at 28 degreesC) extracts of shark cartilage was centrifuged at 6 000 r/min for 30 min and the pellet was discarded, and the supernatant was recentrifuged at 12 000 r/min for 30 min, then ultrafiltrated and fractionally precipitated with 25 %-90 % acetone. The precipitate was applied to a Sephadex G-75 gel column and eluted after the predominant peak of A(280nm) absorbing material with 0.05 mol/L Tris-HCl buffer (containing 0.02 mol/L NaCl and 0.02% NaN3). The material B were composed of three single peptides with molecular mass of 18 000, 15 000 and 12 600, then were purified using reverse-phase high performance liquid chromatography, the eluate was collected, dried and observed by chichen chorioallantoic membrane. The pure inhibitor was homogeneous as a single band on a silver-stained 12.5% sodium dodecyl sulfate-polyacrylamide gel. SCAI-a had a molecular weight of 12 600. It specifically inhibited the chorioallantoic membrane of chick embryos.

• 出版日期2001-9
• 单位中山大学