Determination of reference values for cytokines and matrix metalloproteinases in the synovial fluid of horses of different ages

作者：Chiappe Barbara Maria A; Perrone Gustavo; Caggiano Nicolas; Lastra Yael; Rubatino Florencia; Ferretto Araceli; De Simone Emilio A^*

来源：Veterinarski Arhiv, 2018, 88(2): 159-170.

DOI：10.24099/vet.arhiv.170216

• matrix metalloproteinase
• cytokines
• osteoarthritis
• synovial markers

Osteoarthritis (OA) is the most common joint disease in sport horses. Inflammatory and anti-inflammatory cytokines and metalloproteinases (MMPs) play a crucial role in the degradation of the cartilage extracellular matrix. The study of inflammatory molecular biomarkers could be useful in the diagnosis and choice of therapeutic strategies. However, little is known about the normal values of inflammatory biomarkers at different ages. Moreover, early diagnosis of OA is crucial to ensure early treatment in order to prevent chronic damage. The aim of this study was to establish a reference range for values of pro-inflammatory cytokines (IL-1 beta, IL-4, IL-6 and TNF-alpha) and MMPs (MMP-2 and MMP-9) in the synovial fluid of horses of different ages. The old horses group presented higher values of IL-4 and TNF-alpha than the rest of the groups studied (P<0.001 vs. all other groups). IL-1 beta levels were higher in foals than in all the other groups (P<0.001). No statistically significant differences were found between the groups as regards the mean IL-6 values. Synovial MMP-2 levels were statistically lower in the old horses group (P<0.001) and higher in the foals group (P<0.05). Higher MMP-9 levels were found in the foals group (55.45 +/- 58.1 percentage of proteolysis) (P<0.001 vs. all other groups). This study demonstrates the existence of variations in the levels of pro-inflammatory markers in the synovial fluid of horses at different ages.

• 出版日期2018