Novel role for cystic fibrosis transmembrane conductance regulator in alveolar fluid clearance in lipopolysaccharide-induced acute lung injury in mice

作者：Jin, Weizhong; Song, Yuanlin; Bai, Chunxue; Jiang, Jinjun^*

来源：Respirology, 2013, 18(6): 978-982.

DOI：10.1111/resp.12114

• acute lung injury
• alveolar fluid clearance
• cAMP
• cystic fibrosis transmembrane conductance regulator

Background and objective: Alveolar fluid clearance (AFC) is important for the resolution of acute lung injury (ALI). The role of cystic fibrosis transmembrane conductance regulator (CFTR) in AFC has not been entirely elucidated in animal models of ALI. The aim of this study was to investigate the role of CFTR and its mechanisms in AFC in normal and ALI mice.
Methods: Seventy mice were randomly divided into 14 groups and ALI was established by intratracheal instillation of lipopolysaccharide (LPS). After 48 h, CFTR activator CFTRact-16 or inhibitor CFinh-172 with or without beta-agonist was instillated intratracheally and AFC was measured with radioisotopic tracer.
Results: Although there was no effects of CFTRact-16 on AFC in mice with or without isoproterenol, CFinh-172 markedly decreased isoproterenol-stimulated AFC in both normal (P < 0.01) and LPS-induced ALI mice (P < 0.01) and there was significantly decreased basal AFC in ALI mice (P < 0.05).
Conclusions: These results provide direct functional evidence for CFTR in cAMP-mediated AFC in both normal and ALI mice.

• 出版日期2013-8
• 单位复旦大学; 杭州市第一人民医院; 南京医科大学