An integrated experimental and analytical approach to the chemical state imaging of iron in brain gliomas using X-ray absorption near edge structure spectroscopy

作者：Szczerbowska Boruchowska Magdalena^*; Lankosz Marek; Czyzycki Mateusz; Adamek Dariusz

来源：Analytica Chimica Acta, 2011, 699(2): 153-160.

DOI：10.1016/j.aca.2011.05.044

• Fe oxidation states
• X-ray absorption near edge structure spectroscopy
• Microimaging
• Brain gliomas

X-ray absorption near-edge structure spectroscopy is used for human neoplastic tissues in order to investigate distributions and chemical states of iron. The specimens used in this study were obtained intraoperatively from brain gliomas of different types and various grades of malignancy and from a control subject. An integrated experimental and analytical approach toward topographic and quantitative analysis in thin freeze-dried cryo-sections is presented. The full XANES spectra at the Fe absorption K edge show the presence of both chemical forms of Fe in the analyzed points of the tissues. The main goal of the work is the chemical state imaging of Fe in tissue areas. Topographic analysis of Fe speciation in the tissues investigated with the use of the XANES technique indicates the presence of microstructures where Fe(2+) is dominant as well as those with a high abundance of the oxidized form of Fe. The quantitative analysis shows that for all cases the content of the oxidized form of Fe is significantly higher in comparison with Fe(2+). The highest level of Fe(3+) is found in the control sample, and the lowest one for the glioma of the highest grade of malignancy. The content of either Fe(2+) or Fe(3+) is increased in low grade gliomas in comparison to high-grade malignant tumors.

• 出版日期2011-8-12