Detection of expanded-espectrum-betalactamases by DIRAMIC system: Comparison with the double-disk synergy test and E-test

作者：Espino H Maria; Alvarez Estrella V; Zayas T Angela; Contreras A Rolando

来源：Revista Chilena de Infectologia, 2010, 27(6): 544-550.

• Escherichia coli
• Klebsiella pneumoniae
• extended spectrum betalactamases

The capacity of the DIRAMIC system to detect strains producing extended-spectrum-betalactamase (ESBL) was evaluated through the comparison with two phenotypic confirmatory tests: double-disk synergy test and E-test. Ninety seven clinical isolates of Escherichia coli y Klebsiella spp. previously characterized; not repeated and suspected of being ESBL producers were studied by the three methods. In comparison with E-test and double-disk synergy test, DIRAMIC system showed a sensitivity of 85.7% and 92.7% as well as specificity of 100% and 92.9%; respectively. The values found have a very high degree of concordance (kappa index > 0.80). The results obtained vouch for the utility of the DIRAMIC as a rapid method to alert about the presence of strains producing ESBL enzymes.

• 出版日期2010-12