A Lactobacillus helveticus plasmid detects restriction fragment length polymorphism in different bacterial species

作者：Gancheva AG; Miteva VI^*; Stefanova TT

来源：FEMS Microbiology Letters, 2000, 190(2): 335-339.

• restriction fragment length polymorphism
• plasmid probe
• Lactobacillus helveticus

A small cryptic Lactobacillus helveticus plasmid, pLBL4, was able to reveal restriction fragment length polymorphism in different bacterial species including Lactobacillus species, Bacillus species, and Escherichia coli when used as a DNA probe. The observed polymorphism was a result of the combined hybridization of several microsatellite sequences. The 6-bp sequence (TTGTTT) was repeated 12 times, seven of which were concentrated within the region between 1791 and 1997 bp of the plasmid sequence. The polymorphic patterns generated with pLBL4 differed from those obtained with M13 DNA in the larger number of bands observed. The results presented here open the possibility of using pLBL4 as a new broad-spectrum polymorphic DNA probe for fingerprint analysis.

• 出版日期2000-9-15