Analysis of four sulfonylurea herbicides in cereals using modified Quick, Easy, Cheap, Effective, Rugged, and Safe sample preparation method coupled with liquid chromatography-tandem mass spectrometry

作者：Ni, Yanxia; Yang, Huan; Zhang, Hantong; He, Qiao; Huang, Siqi; Qin, Meiling; Chai, Shuangshuang; Gao, Huanhuan; Ma, Youning^*

来源：Journal of Chromatography A, 2018, 1537: 27-34.

DOI：10.1016/j.chroma.2018.01.017

• Sulfonylurea herbicides
• Primary-secondary amine
• High-performance liquid chromatography-electro-spray ionization-tandem mass spectrometry
• Modified QuEChERS method
• Cereals

A modified Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) sample preparation method, coupled with liquid chromatography-electrospray ionization tandem mass spectrometry, has been developed for the simultaneous analysis of four commonly used sulfonylurea herbicides, ethoxysulfuron, halosulfuron-methyl, mesosulfuron-methyl and orthosulfamuron, in rice, maize, wheat and soybean. Adsorption of the analytes onto the primary-secondary amine used in the clean-up step was avoided by using 1% formic acid in acetonitrile as the extraction solvent to maintain the acidic herbicides in a non ionized state. Trueness studies were carried out at three levels (2.5, 25 and 250 mu g/kg for ethoxysulfuron and 5, 50 and 500 mu g/kg for the other three analytes). Promising trueness (70.2%-119.8%) was achieved for all herbicides in all matrices after clean-up, with relative standard deviations (RSDr) < 18.6%. Satisfactory matrix effects (-19.7% to 14.8%) were also obtained. Good linearity of the calibration curves was achieved, with determination coefficients (r) >= 0.9956, when the concentration of ethoxysulfuron was in the range 0.5-50 mu g/L. and that of the other three analytes was in the range 1.0-500 mu g/L. The RSDWR for within-laboratory reproducibility was 5.7%. The validated method was successfully used to analyze real samples.

• 出版日期2018-2-16
• 单位中国水稻研究所