Nitidine chloride inhibits proliferation and induces apoptosis in ovarian cancer cells by activating the Fas signalling pathway

作者：Chen, Shipeng; Yang, Luo; Feng, Jie^*

来源：Journal of Pharmacy and Pharmacology, 2018, 70(6): 778-786.

DOI：10.1111/jphp.12901

• apoptosis
• Fas signalling pathway
• nitidine chloride
• ovarian cancer

Objectives To explore the apoptotic effects and underlying mechanisms of nitidine chloride (NC) in epithelial ovarian cancer. @@@ Methods The MTT cell proliferation assay was used to detect the inhibitory effects of different concentrations of NC (0, 0.3125, 0.625, 1.25, 2.5, 5 and 10 mu g/ ml) in SKOV3 ovarian carcinoma cells. The number of apoptotic cells was observed by Hoechst staining and measured by flow cytometry. Quantitative PCR was used to measure the expression of Fas, Fas-associated death domain-containing protein (FADD), caspase-8 and caspase-3. RNA interference (RNAi) was used to determine whether caspase-8 played an important role in NC-induced apoptosis. @@@ Key findings Nitidine chloride inhibited the proliferation of SKOV3 cells (IC50 = 2.317 +/- 0.155 mu g/ml) after 24 h of treatment and induced apoptosis (15.9-64.3%). Compared with the control group, a significant increase in Fas, FADD, caspase-8 and caspase-3 gene expression was observed in the NC-treated groups (P < 0.05). After silencing caspase-8 by RNAi, the antiproliferative activity and pro-apoptotic activity of NC in SKOV3 cells decreased (P < 0.05). @@@ Conclusions Our study showed that NC induced apoptosis in SKOV3 cells by activating the Fas signalling pathway, and caspase-8 played an important role in this process.

• 出版日期2018-6
• 单位广西医科大学