A Combination of Enhancer/Silencer Modules Regulates Spatially Restricted Expression of cadherin-7 in Neural Epithelium

作者：Prasad Maneeshi S; Paulson Alicia F^*

来源：Developmental Dynamics, 2011, 240(7): 1756-1768.

DOI：10.1002/dvdy.22675

• cadherin
• gene regulation
• evolutionary conserved regions
• enhancer
• silencer
• electroporation
• transcription factor
• neural epithelium
• embryo

The spatially restricted expression of cadherin-7 to the intermediate domain of the neural epithelium and in migrating neural crest cells during early neural development is potentially regulated by multiple signaling inputs. To identify the regulatory modules involved in regulation of cadherin-7, evolutionary conserved non-coding sequences in the cadherin-7 locus were analyzed. This led to the identification of an evolutionary conserved region of 606 bp (ECR1) that together with the cadherin-7 promoter recapitulates endogenous cadherin-7 expression in intermediate neural tube, spinal motor neurons, interneurons, and dorsal root ganglia. Deletion analysis of ECR1 revealed a 19-bp block that is essential for ECR1 enhancer activity, while two separate blocks of 10 and 12 bp were found to be essential for ECR1 silencer activity in the dorsal and ventral neural epithelium, respectively. Together, these data provide an insight into tissue-specific regulatory regions that might be involved in regulation of cadherin-7 gene expression. Developmental Dynamics 240:1756-1768, 2011.

• 出版日期2011-7