A rapid, simple, and accurate plaque assay for human respiratory syncytial virus (HRSV)

作者：Kim, Kyung Sook; Kim, Ah-Ra; Piao, Ying; Lee, Ju-Hie; Quan, Fu-Shi^*

来源：Journal of Immunological Methods, 2017, 446: 15-20.

DOI：10.1016/j.jim.2017.03.020

• Human respiratory syncytial virus
• Immunostaining
• Plaque assay

Plaque assays of human respiratory syncytial virus (HRSV) are time-consuming, requiring 4 to 7 days for plaque formation and several hours for dye staining. Here, we describe a simple method by which RSV plaques can be visualized and counted with the naked eye only 2 days after infection of HEp-2 cells. In this assay, the infected cells are stained with monoclonal antibodies and the plaques are developed using diaminobenzidine (DAB). We tested the accuracy of this new plaque assay by comparing the results obtained on days 1, 2, 3, 4, 5, and 6 post-infection. The whole procedure is significantly simpler than the traditional method, with an immunostaining process of around 1.5 h. Our method is rapid, accurate, and simple; thus, it has the potential to significantly contribute to studies related to RSV disease.

• 出版日期2017-7
• 单位延边大学