Pharmacokinetics Study of the Antitumor Drug CYC-116 in Rat Plasma by Using LC-MS Analysis

作者：Su, Jing; Chen, Xiaohui; Li, Qing; Yu, Zhiguo; Guan, Xiaoduo; Geng, Lulu; Bi, Kaishun^*

来源：Chromatographia, 2012, 75(5-6): 263-268.

DOI：10.1007/s10337-011-2175-3

• LC-MS
• Anticancer agent
• CYC-116
• Pharmacokinetics

A sensitive and rapid liquid chromatography-mass spectrometry (LC-MS) assay was established for the quantitation of CYC-116, an antitumor drug, in rat plasma. The chromatographic separation was accomplished on a Kromasil C-18 column (150 mm x 4.6 mm i.d., 5 mu m particle size) at an isocratic flow rate of 0.8 mL min(-1) using acetonitrile-water-formic acid (23.5:76.5:0.1, v/v/v) as the mobile phase. The plasma extraction was performed by liquid-liquid extraction using ethyl acetate as the solvent, and the extracts were subjected to MS analysis using a quadrupole mass spectrometer. The calibration curve of CYC-116 was linear over the concentration range of 5-2,500 ng mL(-1) (r = 0.9955). The mean recovery was 85.0 +/- A 8.0%, and the matrix effect ranged from 90.0 to 110.0%. The intra- and interday precisions were less than 11.8 and 6.6%, respectively, and the accuracy was within +/- 5.8%. The method was successfully applied to study the pharmacokinetics of CYC-116 in rats after oral administration.

• 出版日期2012-3
• 单位沈阳药科大学