Analysis of protein-protein interaction between late cornified envelope proteins and corneodesmosin

作者：Bergboer Judith G M; Dulak Maria G; van Vlijmen Willems Ivonne M J J; Jonca Nathalie; van Wijk Erwin; Hendriks Wiljan J A J; Zeeuwen Patrick L J M; Schalkwijk Joost^*

来源：Experimental Dermatology, 2014, 23(10): 769-771.

DOI：10.1111/exd.12524

• corneodesmosin
• HLA-C
• keratinocytes
• late cornified envelope
• psoriasis

Deletion of two members of the late cornified envelope (LCE) family, LCE3B and LCE3C (LCE3C_LCE3B-del), has been identified as risk factor for psoriasis with a possible role in skin barrier function. Moreover, genetic interaction between LCE3C_LCE3B-del and HLA-C*06, located in the psoriasis susceptibility regions 4 and 1 (PSORS4 and 1), has been reported in several populations. Because of high linkage disequilibrium between the PSORS1 genes HLA-C*06 and corneodesmosin (CDSN), both genes are potentially involved in psoriasis. As corneodesmosin and LCE proteins are both constituents of the stratum corneum, we investigated potential direct protein-protein interactions between six LCE proteins and two corneodesmosin sequence variants. Partial colocalization of LCE2 and CDSN was observed in normal and psoriasis skin using immunofluorescence microscopy. Co-expression of eCFP-LCE and mRFP-CDSN proteins in COS-1 cells and human adult keratinocytes, and GST pull-down results did not provide evidence for direct interactions between LCE proteins and CDSN variants.

• 出版日期2014-10